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quantstudio real-time fluorescence quantitative pcr instrument  (Thermo Fisher)


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    Thermo Fisher quantstudio real-time fluorescence quantitative pcr instrument
    Quantstudio Real Time Fluorescence Quantitative Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quantstudio%C2%AE+5+real-time+pcr+instrument/quantstudio+3+real+time+pcr+system/med_rxiv__2025__07__13__25331478-100-7-13
    Average 90 stars, based on 1 article reviews
    quantstudio real-time fluorescence quantitative pcr instrument - by Bioz Stars, 2026-09
    90/100 stars

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    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Glutathione S-transferases GhGSTF1 and GhGSTF2 involved in the anthocyanin accumulation in Gossypium hirsutum L.
    Article Snippet: International Journal of Biological Macromolecules 165 (2020) 2565–2575 Contents lists available at ScienceDirect International Journal of Biological Macromolecules j ourna l homepage: ht tp : / /www.e lsev ie r .com/ locate / i j b iomac Glutathione S-transferases GhGSTF1 and GhGSTF2 involved in the anthocyanin accumulation in Gossypium hirsutum L. Shuyan Li a,b,c,d, Dongyun Zuo b, Hailiang Cheng b, Mushtaque Ali b, Chaofeng Wu a,d, Javaria Ashraf b, Youping Zhang b, Xiaoxu Feng b, Zhongxu Lin c, Qiaolian Wang b, Limin Lv b, Guoli Song a,b,⁎ a Research Base, Anyang Institute of Technology, State Key Laboratory of Cotton Biology, Anyang 455000, Henan, China b Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang 455000, Henan, China c Huazhong Agricultural University, Wuhan 430070, Hubei, China d Anyang Institute of Technology, Anyang 455000, Henan, China ⁎ Corresponding author at: Research Base, Anyang Ins Laboratory of Cotton Biology, Anyang 455000, Henan, Chi E-mail address: sglzms@163.com (G. Song). https://doi.org/10.1016/j.ijbiomac.2020.10.101 0141-8130/© 2020 Elsevier B.V. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 12 August 2020 Received in revised form 10 October 2020 Accepted 14 October 2020 Available online 22 October 2020 Keywords: Anthocyanin Glutathione S-transferase GhGSTF The glutathione S-transferases (GSTs) are important enzymes of secondary metabolism in plants.. In this study, two putative GSTs, GhGSTF1 and GhGSTF2, were identified as anthocyanin-related GSTs by the transcriptome data of the leaves of Gossypium hirsutum L. TM-1 and T586.. The quantitative real-time PCR showed that GhGSTF1 and GhGSTF2were highly expressed in red leaves and stems of Gossypium hirsutum L. T586.

    Article Title: Nano chitosan–zinc complex improves the growth performance and antioxidant capacity of the small intestine in weaned piglets
    Article Snippet: The present study was conducted to test the hypothesis that dietary supplementation with a nano chitosan-zinc complex (CP-Zn, 100 mg/kg zinc) could alleviate weaning stress in piglets challenged with ETEC K88 by improving growth performance and intestinal antioxidant capacity.. The in vivo effects of CP-Zn on growth performance variables (including gastrointestinal digestion and absorption functions and the levels of key proteins related to muscle growth) and the antioxidant capacity of the small intestine were evaluated in 72 weaned piglets.. The porcine jejunal epithelial cell line IPEC-J2 was used to further investigate the antioxidant mechanism of CP-Zn in vitro.

    Article Title: Effects of maternal T-2 toxin exposure on microorganisms and intestinal barrier function in young mice.
    Article Snippet: Real-time PCR was conducted on the QuantStudio® 5 Real-Time PCR Instrument (Applied Biosystems, Thermo Fisher Scientific, Wilmington, DE, USA) with the SYBR Green I (TSINGKE BioTech Co., Ltd., Beijing, China).

    Article Title: BST2 regulated by the transcription factor STAT1 can promote metastasis, invasion and proliferation of oral squamous cell carcinoma via the AKT/ERK1/2 signaling pathway.
    Article Snippet: Quantitative PCR assays were performed using a A28134 QuantStudio® 5 Real‐Time PCR Instrument (Thermo Fisher Scientific, Inc.) and iTaq Universal SYBR Green Supermixi reagent (Bio‐Rad Laboratories, Inc.) in 8 Strip Real‐time PCR Tubes (Sangon Biotech Co., Ltd.).

    Article Title: Fine particulate matter from pig house induced immune response by activating TLR4/MAPK/NF-κB pathway and NLRP3 inflammasome in alveolar macrophages.
    Article Snippet: The concentration, morphology and chemical components of PM2.5 were investigated.. PM2.5 can activate TLR4 and NLRP3 inflammasome in 3D4/21 cells.. MAPK and NF-kB pathways participated in NLRP3 inflammasome

    Article Title: The effects of indole-2,3-dione on radiosensitivity of U251 cells and zebrafish embryos.
    Article Snippet: Radiotherapy is an effective treatment for gliomas, which are among the most malignant tumors in the central nervous system (CNS).. A significant challenge in glioma radiotherapy is improving tumor control while minimizing damage to normal nervous tissue.. In this study, we investigated the dual role of indole-2,3-dione (Isatin, ISA), a compound with diverse biological and pharmacological activities, including anticancer, antioxidant, and anti-inflammatory properties, in modulating radiosensitivity.

    Article Title: Combined transcriptome and metabolome analysis provides insight into the ERF073 - malic acid network in pakchoi under submergence stress
    Article Snippet: The PCR reaction with TransStart ® Top Green qPCR SuperMix (+ Dye I) (Transgene, China) was carried out using a QuantStudio® 5 Real-time PCR Instrument (Thermo Fisher Scientific).

    Real-time Polymerase Chain Reaction:

    Article Title: Glutathione S-transferases GhGSTF1 and GhGSTF2 involved in the anthocyanin accumulation in Gossypium hirsutum L.
    Article Snippet: International Journal of Biological Macromolecules 165 (2020) 2565–2575 Contents lists available at ScienceDirect International Journal of Biological Macromolecules j ourna l homepage: ht tp : / /www.e lsev ie r .com/ locate / i j b iomac Glutathione S-transferases GhGSTF1 and GhGSTF2 involved in the anthocyanin accumulation in Gossypium hirsutum L. Shuyan Li a,b,c,d, Dongyun Zuo b, Hailiang Cheng b, Mushtaque Ali b, Chaofeng Wu a,d, Javaria Ashraf b, Youping Zhang b, Xiaoxu Feng b, Zhongxu Lin c, Qiaolian Wang b, Limin Lv b, Guoli Song a,b,⁎ a Research Base, Anyang Institute of Technology, State Key Laboratory of Cotton Biology, Anyang 455000, Henan, China b Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang 455000, Henan, China c Huazhong Agricultural University, Wuhan 430070, Hubei, China d Anyang Institute of Technology, Anyang 455000, Henan, China ⁎ Corresponding author at: Research Base, Anyang Ins Laboratory of Cotton Biology, Anyang 455000, Henan, Chi E-mail address: sglzms@163.com (G. Song). https://doi.org/10.1016/j.ijbiomac.2020.10.101 0141-8130/© 2020 Elsevier B.V. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 12 August 2020 Received in revised form 10 October 2020 Accepted 14 October 2020 Available online 22 October 2020 Keywords: Anthocyanin Glutathione S-transferase GhGSTF The glutathione S-transferases (GSTs) are important enzymes of secondary metabolism in plants.. In this study, two putative GSTs, GhGSTF1 and GhGSTF2, were identified as anthocyanin-related GSTs by the transcriptome data of the leaves of Gossypium hirsutum L. TM-1 and T586.. The quantitative real-time PCR showed that GhGSTF1 and GhGSTF2were highly expressed in red leaves and stems of Gossypium hirsutum L. T586.

    Article Title: Nano chitosan–zinc complex improves the growth performance and antioxidant capacity of the small intestine in weaned piglets
    Article Snippet: The present study was conducted to test the hypothesis that dietary supplementation with a nano chitosan-zinc complex (CP-Zn, 100 mg/kg zinc) could alleviate weaning stress in piglets challenged with ETEC K88 by improving growth performance and intestinal antioxidant capacity.. The in vivo effects of CP-Zn on growth performance variables (including gastrointestinal digestion and absorption functions and the levels of key proteins related to muscle growth) and the antioxidant capacity of the small intestine were evaluated in 72 weaned piglets.. The porcine jejunal epithelial cell line IPEC-J2 was used to further investigate the antioxidant mechanism of CP-Zn in vitro.

    Article Title: Effects of maternal T-2 toxin exposure on microorganisms and intestinal barrier function in young mice.
    Article Snippet: Real-time PCR was conducted on the QuantStudio® 5 Real-Time PCR Instrument (Applied Biosystems, Thermo Fisher Scientific, Wilmington, DE, USA) with the SYBR Green I (TSINGKE BioTech Co., Ltd., Beijing, China).

    Article Title: BST2 regulated by the transcription factor STAT1 can promote metastasis, invasion and proliferation of oral squamous cell carcinoma via the AKT/ERK1/2 signaling pathway.
    Article Snippet: Quantitative PCR assays were performed using a A28134 QuantStudio® 5 Real‐Time PCR Instrument (Thermo Fisher Scientific, Inc.) and iTaq Universal SYBR Green Supermixi reagent (Bio‐Rad Laboratories, Inc.) in 8 Strip Real‐time PCR Tubes (Sangon Biotech Co., Ltd.).

    Article Title: Fine particulate matter from pig house induced immune response by activating TLR4/MAPK/NF-κB pathway and NLRP3 inflammasome in alveolar macrophages.
    Article Snippet: The concentration, morphology and chemical components of PM2.5 were investigated.. PM2.5 can activate TLR4 and NLRP3 inflammasome in 3D4/21 cells.. MAPK and NF-kB pathways participated in NLRP3 inflammasome

    Article Title: The effects of indole-2,3-dione on radiosensitivity of U251 cells and zebrafish embryos.
    Article Snippet: Radiotherapy is an effective treatment for gliomas, which are among the most malignant tumors in the central nervous system (CNS).. A significant challenge in glioma radiotherapy is improving tumor control while minimizing damage to normal nervous tissue.. In this study, we investigated the dual role of indole-2,3-dione (Isatin, ISA), a compound with diverse biological and pharmacological activities, including anticancer, antioxidant, and anti-inflammatory properties, in modulating radiosensitivity.

    Article Title: Combined transcriptome and metabolome analysis provides insight into the ERF073 - malic acid network in pakchoi under submergence stress
    Article Snippet: The PCR reaction with TransStart ® Top Green qPCR SuperMix (+ Dye I) (Transgene, China) was carried out using a QuantStudio® 5 Real-time PCR Instrument (Thermo Fisher Scientific).

    Gene Expression:

    Article Title: Glutathione S-transferases GhGSTF1 and GhGSTF2 involved in the anthocyanin accumulation in Gossypium hirsutum L.
    Article Snippet: International Journal of Biological Macromolecules 165 (2020) 2565–2575 Contents lists available at ScienceDirect International Journal of Biological Macromolecules j ourna l homepage: ht tp : / /www.e lsev ie r .com/ locate / i j b iomac Glutathione S-transferases GhGSTF1 and GhGSTF2 involved in the anthocyanin accumulation in Gossypium hirsutum L. Shuyan Li a,b,c,d, Dongyun Zuo b, Hailiang Cheng b, Mushtaque Ali b, Chaofeng Wu a,d, Javaria Ashraf b, Youping Zhang b, Xiaoxu Feng b, Zhongxu Lin c, Qiaolian Wang b, Limin Lv b, Guoli Song a,b,⁎ a Research Base, Anyang Institute of Technology, State Key Laboratory of Cotton Biology, Anyang 455000, Henan, China b Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang 455000, Henan, China c Huazhong Agricultural University, Wuhan 430070, Hubei, China d Anyang Institute of Technology, Anyang 455000, Henan, China ⁎ Corresponding author at: Research Base, Anyang Ins Laboratory of Cotton Biology, Anyang 455000, Henan, Chi E-mail address: sglzms@163.com (G. Song). https://doi.org/10.1016/j.ijbiomac.2020.10.101 0141-8130/© 2020 Elsevier B.V. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 12 August 2020 Received in revised form 10 October 2020 Accepted 14 October 2020 Available online 22 October 2020 Keywords: Anthocyanin Glutathione S-transferase GhGSTF The glutathione S-transferases (GSTs) are important enzymes of secondary metabolism in plants.. In this study, two putative GSTs, GhGSTF1 and GhGSTF2, were identified as anthocyanin-related GSTs by the transcriptome data of the leaves of Gossypium hirsutum L. TM-1 and T586.. The quantitative real-time PCR showed that GhGSTF1 and GhGSTF2were highly expressed in red leaves and stems of Gossypium hirsutum L. T586.

    Article Title: Nano chitosan–zinc complex improves the growth performance and antioxidant capacity of the small intestine in weaned piglets
    Article Snippet: The present study was conducted to test the hypothesis that dietary supplementation with a nano chitosan-zinc complex (CP-Zn, 100 mg/kg zinc) could alleviate weaning stress in piglets challenged with ETEC K88 by improving growth performance and intestinal antioxidant capacity.. The in vivo effects of CP-Zn on growth performance variables (including gastrointestinal digestion and absorption functions and the levels of key proteins related to muscle growth) and the antioxidant capacity of the small intestine were evaluated in 72 weaned piglets.. The porcine jejunal epithelial cell line IPEC-J2 was used to further investigate the antioxidant mechanism of CP-Zn in vitro.

    Article Title: Effects of maternal T-2 toxin exposure on microorganisms and intestinal barrier function in young mice.
    Article Snippet: Real-time PCR was conducted on the QuantStudio® 5 Real-Time PCR Instrument (Applied Biosystems, Thermo Fisher Scientific, Wilmington, DE, USA) with the SYBR Green I (TSINGKE BioTech Co., Ltd., Beijing, China).

    Article Title: BST2 regulated by the transcription factor STAT1 can promote metastasis, invasion and proliferation of oral squamous cell carcinoma via the AKT/ERK1/2 signaling pathway.
    Article Snippet: Quantitative PCR assays were performed using a A28134 QuantStudio® 5 Real‐Time PCR Instrument (Thermo Fisher Scientific, Inc.) and iTaq Universal SYBR Green Supermixi reagent (Bio‐Rad Laboratories, Inc.) in 8 Strip Real‐time PCR Tubes (Sangon Biotech Co., Ltd.).

    Article Title: Fine particulate matter from pig house induced immune response by activating TLR4/MAPK/NF-κB pathway and NLRP3 inflammasome in alveolar macrophages.
    Article Snippet: The concentration, morphology and chemical components of PM2.5 were investigated.. PM2.5 can activate TLR4 and NLRP3 inflammasome in 3D4/21 cells.. MAPK and NF-kB pathways participated in NLRP3 inflammasome

    Article Title: The effects of indole-2,3-dione on radiosensitivity of U251 cells and zebrafish embryos.
    Article Snippet: Radiotherapy is an effective treatment for gliomas, which are among the most malignant tumors in the central nervous system (CNS).. A significant challenge in glioma radiotherapy is improving tumor control while minimizing damage to normal nervous tissue.. In this study, we investigated the dual role of indole-2,3-dione (Isatin, ISA), a compound with diverse biological and pharmacological activities, including anticancer, antioxidant, and anti-inflammatory properties, in modulating radiosensitivity.

    Article Title: Combined transcriptome and metabolome analysis provides insight into the ERF073 - malic acid network in pakchoi under submergence stress
    Article Snippet: The PCR reaction with TransStart ® Top Green qPCR SuperMix (+ Dye I) (Transgene, China) was carried out using a QuantStudio® 5 Real-time PCR Instrument (Thermo Fisher Scientific).

    Polymerase Chain Reaction:

    Article Title: Glutathione S-transferases GhGSTF1 and GhGSTF2 involved in the anthocyanin accumulation in Gossypium hirsutum L.
    Article Snippet: International Journal of Biological Macromolecules 165 (2020) 2565–2575 Contents lists available at ScienceDirect International Journal of Biological Macromolecules j ourna l homepage: ht tp : / /www.e lsev ie r .com/ locate / i j b iomac Glutathione S-transferases GhGSTF1 and GhGSTF2 involved in the anthocyanin accumulation in Gossypium hirsutum L. Shuyan Li a,b,c,d, Dongyun Zuo b, Hailiang Cheng b, Mushtaque Ali b, Chaofeng Wu a,d, Javaria Ashraf b, Youping Zhang b, Xiaoxu Feng b, Zhongxu Lin c, Qiaolian Wang b, Limin Lv b, Guoli Song a,b,⁎ a Research Base, Anyang Institute of Technology, State Key Laboratory of Cotton Biology, Anyang 455000, Henan, China b Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang 455000, Henan, China c Huazhong Agricultural University, Wuhan 430070, Hubei, China d Anyang Institute of Technology, Anyang 455000, Henan, China ⁎ Corresponding author at: Research Base, Anyang Ins Laboratory of Cotton Biology, Anyang 455000, Henan, Chi E-mail address: sglzms@163.com (G. Song). https://doi.org/10.1016/j.ijbiomac.2020.10.101 0141-8130/© 2020 Elsevier B.V. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 12 August 2020 Received in revised form 10 October 2020 Accepted 14 October 2020 Available online 22 October 2020 Keywords: Anthocyanin Glutathione S-transferase GhGSTF The glutathione S-transferases (GSTs) are important enzymes of secondary metabolism in plants.. In this study, two putative GSTs, GhGSTF1 and GhGSTF2, were identified as anthocyanin-related GSTs by the transcriptome data of the leaves of Gossypium hirsutum L. TM-1 and T586.. The quantitative real-time PCR showed that GhGSTF1 and GhGSTF2were highly expressed in red leaves and stems of Gossypium hirsutum L. T586.

    Article Title: Nano chitosan–zinc complex improves the growth performance and antioxidant capacity of the small intestine in weaned piglets
    Article Snippet: The present study was conducted to test the hypothesis that dietary supplementation with a nano chitosan-zinc complex (CP-Zn, 100 mg/kg zinc) could alleviate weaning stress in piglets challenged with ETEC K88 by improving growth performance and intestinal antioxidant capacity.. The in vivo effects of CP-Zn on growth performance variables (including gastrointestinal digestion and absorption functions and the levels of key proteins related to muscle growth) and the antioxidant capacity of the small intestine were evaluated in 72 weaned piglets.. The porcine jejunal epithelial cell line IPEC-J2 was used to further investigate the antioxidant mechanism of CP-Zn in vitro.

    Article Title: Effects of maternal T-2 toxin exposure on microorganisms and intestinal barrier function in young mice.
    Article Snippet: Real-time PCR was conducted on the QuantStudio® 5 Real-Time PCR Instrument (Applied Biosystems, Thermo Fisher Scientific, Wilmington, DE, USA) with the SYBR Green I (TSINGKE BioTech Co., Ltd., Beijing, China).

    Article Title: BST2 regulated by the transcription factor STAT1 can promote metastasis, invasion and proliferation of oral squamous cell carcinoma via the AKT/ERK1/2 signaling pathway.
    Article Snippet: Quantitative PCR assays were performed using a A28134 QuantStudio® 5 Real‐Time PCR Instrument (Thermo Fisher Scientific, Inc.) and iTaq Universal SYBR Green Supermixi reagent (Bio‐Rad Laboratories, Inc.) in 8 Strip Real‐time PCR Tubes (Sangon Biotech Co., Ltd.).

    Article Title: Fine particulate matter from pig house induced immune response by activating TLR4/MAPK/NF-κB pathway and NLRP3 inflammasome in alveolar macrophages.
    Article Snippet: The concentration, morphology and chemical components of PM2.5 were investigated.. PM2.5 can activate TLR4 and NLRP3 inflammasome in 3D4/21 cells.. MAPK and NF-kB pathways participated in NLRP3 inflammasome

    Article Title: The effects of indole-2,3-dione on radiosensitivity of U251 cells and zebrafish embryos.
    Article Snippet: Radiotherapy is an effective treatment for gliomas, which are among the most malignant tumors in the central nervous system (CNS).. A significant challenge in glioma radiotherapy is improving tumor control while minimizing damage to normal nervous tissue.. In this study, we investigated the dual role of indole-2,3-dione (Isatin, ISA), a compound with diverse biological and pharmacological activities, including anticancer, antioxidant, and anti-inflammatory properties, in modulating radiosensitivity.

    Article Title: Combined transcriptome and metabolome analysis provides insight into the ERF073 - malic acid network in pakchoi under submergence stress
    Article Snippet: The PCR reaction with TransStart ® Top Green qPCR SuperMix (+ Dye I) (Transgene, China) was carried out using a QuantStudio® 5 Real-time PCR Instrument (Thermo Fisher Scientific).

    SYBR Green Assay:

    Article Title: Glutathione S-transferases GhGSTF1 and GhGSTF2 involved in the anthocyanin accumulation in Gossypium hirsutum L.
    Article Snippet: International Journal of Biological Macromolecules 165 (2020) 2565–2575 Contents lists available at ScienceDirect International Journal of Biological Macromolecules j ourna l homepage: ht tp : / /www.e lsev ie r .com/ locate / i j b iomac Glutathione S-transferases GhGSTF1 and GhGSTF2 involved in the anthocyanin accumulation in Gossypium hirsutum L. Shuyan Li a,b,c,d, Dongyun Zuo b, Hailiang Cheng b, Mushtaque Ali b, Chaofeng Wu a,d, Javaria Ashraf b, Youping Zhang b, Xiaoxu Feng b, Zhongxu Lin c, Qiaolian Wang b, Limin Lv b, Guoli Song a,b,⁎ a Research Base, Anyang Institute of Technology, State Key Laboratory of Cotton Biology, Anyang 455000, Henan, China b Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang 455000, Henan, China c Huazhong Agricultural University, Wuhan 430070, Hubei, China d Anyang Institute of Technology, Anyang 455000, Henan, China ⁎ Corresponding author at: Research Base, Anyang Ins Laboratory of Cotton Biology, Anyang 455000, Henan, Chi E-mail address: sglzms@163.com (G. Song). https://doi.org/10.1016/j.ijbiomac.2020.10.101 0141-8130/© 2020 Elsevier B.V. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 12 August 2020 Received in revised form 10 October 2020 Accepted 14 October 2020 Available online 22 October 2020 Keywords: Anthocyanin Glutathione S-transferase GhGSTF The glutathione S-transferases (GSTs) are important enzymes of secondary metabolism in plants.. In this study, two putative GSTs, GhGSTF1 and GhGSTF2, were identified as anthocyanin-related GSTs by the transcriptome data of the leaves of Gossypium hirsutum L. TM-1 and T586.. The quantitative real-time PCR showed that GhGSTF1 and GhGSTF2were highly expressed in red leaves and stems of Gossypium hirsutum L. T586.

    Article Title: Nano chitosan–zinc complex improves the growth performance and antioxidant capacity of the small intestine in weaned piglets
    Article Snippet: The present study was conducted to test the hypothesis that dietary supplementation with a nano chitosan-zinc complex (CP-Zn, 100 mg/kg zinc) could alleviate weaning stress in piglets challenged with ETEC K88 by improving growth performance and intestinal antioxidant capacity.. The in vivo effects of CP-Zn on growth performance variables (including gastrointestinal digestion and absorption functions and the levels of key proteins related to muscle growth) and the antioxidant capacity of the small intestine were evaluated in 72 weaned piglets.. The porcine jejunal epithelial cell line IPEC-J2 was used to further investigate the antioxidant mechanism of CP-Zn in vitro.

    Article Title: Effects of maternal T-2 toxin exposure on microorganisms and intestinal barrier function in young mice.
    Article Snippet: Real-time PCR was conducted on the QuantStudio® 5 Real-Time PCR Instrument (Applied Biosystems, Thermo Fisher Scientific, Wilmington, DE, USA) with the SYBR Green I (TSINGKE BioTech Co., Ltd., Beijing, China).

    Article Title: BST2 regulated by the transcription factor STAT1 can promote metastasis, invasion and proliferation of oral squamous cell carcinoma via the AKT/ERK1/2 signaling pathway.
    Article Snippet: Quantitative PCR assays were performed using a A28134 QuantStudio® 5 Real‐Time PCR Instrument (Thermo Fisher Scientific, Inc.) and iTaq Universal SYBR Green Supermixi reagent (Bio‐Rad Laboratories, Inc.) in 8 Strip Real‐time PCR Tubes (Sangon Biotech Co., Ltd.).

    Article Title: Fine particulate matter from pig house induced immune response by activating TLR4/MAPK/NF-κB pathway and NLRP3 inflammasome in alveolar macrophages.
    Article Snippet: The concentration, morphology and chemical components of PM2.5 were investigated.. PM2.5 can activate TLR4 and NLRP3 inflammasome in 3D4/21 cells.. MAPK and NF-kB pathways participated in NLRP3 inflammasome

    Article Title: The effects of indole-2,3-dione on radiosensitivity of U251 cells and zebrafish embryos.
    Article Snippet: Radiotherapy is an effective treatment for gliomas, which are among the most malignant tumors in the central nervous system (CNS).. A significant challenge in glioma radiotherapy is improving tumor control while minimizing damage to normal nervous tissue.. In this study, we investigated the dual role of indole-2,3-dione (Isatin, ISA), a compound with diverse biological and pharmacological activities, including anticancer, antioxidant, and anti-inflammatory properties, in modulating radiosensitivity.

    Article Title: Combined transcriptome and metabolome analysis provides insight into the ERF073 - malic acid network in pakchoi under submergence stress
    Article Snippet: The PCR reaction with TransStart ® Top Green qPCR SuperMix (+ Dye I) (Transgene, China) was carried out using a QuantStudio® 5 Real-time PCR Instrument (Thermo Fisher Scientific).

    Stripping Membranes:

    Article Title: Glutathione S-transferases GhGSTF1 and GhGSTF2 involved in the anthocyanin accumulation in Gossypium hirsutum L.
    Article Snippet: International Journal of Biological Macromolecules 165 (2020) 2565–2575 Contents lists available at ScienceDirect International Journal of Biological Macromolecules j ourna l homepage: ht tp : / /www.e lsev ie r .com/ locate / i j b iomac Glutathione S-transferases GhGSTF1 and GhGSTF2 involved in the anthocyanin accumulation in Gossypium hirsutum L. Shuyan Li a,b,c,d, Dongyun Zuo b, Hailiang Cheng b, Mushtaque Ali b, Chaofeng Wu a,d, Javaria Ashraf b, Youping Zhang b, Xiaoxu Feng b, Zhongxu Lin c, Qiaolian Wang b, Limin Lv b, Guoli Song a,b,⁎ a Research Base, Anyang Institute of Technology, State Key Laboratory of Cotton Biology, Anyang 455000, Henan, China b Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang 455000, Henan, China c Huazhong Agricultural University, Wuhan 430070, Hubei, China d Anyang Institute of Technology, Anyang 455000, Henan, China ⁎ Corresponding author at: Research Base, Anyang Ins Laboratory of Cotton Biology, Anyang 455000, Henan, Chi E-mail address: sglzms@163.com (G. Song). https://doi.org/10.1016/j.ijbiomac.2020.10.101 0141-8130/© 2020 Elsevier B.V. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 12 August 2020 Received in revised form 10 October 2020 Accepted 14 October 2020 Available online 22 October 2020 Keywords: Anthocyanin Glutathione S-transferase GhGSTF The glutathione S-transferases (GSTs) are important enzymes of secondary metabolism in plants.. In this study, two putative GSTs, GhGSTF1 and GhGSTF2, were identified as anthocyanin-related GSTs by the transcriptome data of the leaves of Gossypium hirsutum L. TM-1 and T586.. The quantitative real-time PCR showed that GhGSTF1 and GhGSTF2were highly expressed in red leaves and stems of Gossypium hirsutum L. T586.

    Article Title: Nano chitosan–zinc complex improves the growth performance and antioxidant capacity of the small intestine in weaned piglets
    Article Snippet: The present study was conducted to test the hypothesis that dietary supplementation with a nano chitosan-zinc complex (CP-Zn, 100 mg/kg zinc) could alleviate weaning stress in piglets challenged with ETEC K88 by improving growth performance and intestinal antioxidant capacity.. The in vivo effects of CP-Zn on growth performance variables (including gastrointestinal digestion and absorption functions and the levels of key proteins related to muscle growth) and the antioxidant capacity of the small intestine were evaluated in 72 weaned piglets.. The porcine jejunal epithelial cell line IPEC-J2 was used to further investigate the antioxidant mechanism of CP-Zn in vitro.

    Article Title: Effects of maternal T-2 toxin exposure on microorganisms and intestinal barrier function in young mice.
    Article Snippet: Real-time PCR was conducted on the QuantStudio® 5 Real-Time PCR Instrument (Applied Biosystems, Thermo Fisher Scientific, Wilmington, DE, USA) with the SYBR Green I (TSINGKE BioTech Co., Ltd., Beijing, China).

    Article Title: BST2 regulated by the transcription factor STAT1 can promote metastasis, invasion and proliferation of oral squamous cell carcinoma via the AKT/ERK1/2 signaling pathway.
    Article Snippet: Quantitative PCR assays were performed using a A28134 QuantStudio® 5 Real‐Time PCR Instrument (Thermo Fisher Scientific, Inc.) and iTaq Universal SYBR Green Supermixi reagent (Bio‐Rad Laboratories, Inc.) in 8 Strip Real‐time PCR Tubes (Sangon Biotech Co., Ltd.).

    Article Title: Fine particulate matter from pig house induced immune response by activating TLR4/MAPK/NF-κB pathway and NLRP3 inflammasome in alveolar macrophages.
    Article Snippet: The concentration, morphology and chemical components of PM2.5 were investigated.. PM2.5 can activate TLR4 and NLRP3 inflammasome in 3D4/21 cells.. MAPK and NF-kB pathways participated in NLRP3 inflammasome

    Article Title: The effects of indole-2,3-dione on radiosensitivity of U251 cells and zebrafish embryos.
    Article Snippet: Radiotherapy is an effective treatment for gliomas, which are among the most malignant tumors in the central nervous system (CNS).. A significant challenge in glioma radiotherapy is improving tumor control while minimizing damage to normal nervous tissue.. In this study, we investigated the dual role of indole-2,3-dione (Isatin, ISA), a compound with diverse biological and pharmacological activities, including anticancer, antioxidant, and anti-inflammatory properties, in modulating radiosensitivity.

    Article Title: Combined transcriptome and metabolome analysis provides insight into the ERF073 - malic acid network in pakchoi under submergence stress
    Article Snippet: The PCR reaction with TransStart ® Top Green qPCR SuperMix (+ Dye I) (Transgene, China) was carried out using a QuantStudio® 5 Real-time PCR Instrument (Thermo Fisher Scientific).

    Quantitative RT-PCR:

    Article Title: Glutathione S-transferases GhGSTF1 and GhGSTF2 involved in the anthocyanin accumulation in Gossypium hirsutum L.
    Article Snippet: International Journal of Biological Macromolecules 165 (2020) 2565–2575 Contents lists available at ScienceDirect International Journal of Biological Macromolecules j ourna l homepage: ht tp : / /www.e lsev ie r .com/ locate / i j b iomac Glutathione S-transferases GhGSTF1 and GhGSTF2 involved in the anthocyanin accumulation in Gossypium hirsutum L. Shuyan Li a,b,c,d, Dongyun Zuo b, Hailiang Cheng b, Mushtaque Ali b, Chaofeng Wu a,d, Javaria Ashraf b, Youping Zhang b, Xiaoxu Feng b, Zhongxu Lin c, Qiaolian Wang b, Limin Lv b, Guoli Song a,b,⁎ a Research Base, Anyang Institute of Technology, State Key Laboratory of Cotton Biology, Anyang 455000, Henan, China b Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang 455000, Henan, China c Huazhong Agricultural University, Wuhan 430070, Hubei, China d Anyang Institute of Technology, Anyang 455000, Henan, China ⁎ Corresponding author at: Research Base, Anyang Ins Laboratory of Cotton Biology, Anyang 455000, Henan, Chi E-mail address: sglzms@163.com (G. Song). https://doi.org/10.1016/j.ijbiomac.2020.10.101 0141-8130/© 2020 Elsevier B.V. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 12 August 2020 Received in revised form 10 October 2020 Accepted 14 October 2020 Available online 22 October 2020 Keywords: Anthocyanin Glutathione S-transferase GhGSTF The glutathione S-transferases (GSTs) are important enzymes of secondary metabolism in plants.. In this study, two putative GSTs, GhGSTF1 and GhGSTF2, were identified as anthocyanin-related GSTs by the transcriptome data of the leaves of Gossypium hirsutum L. TM-1 and T586.. The quantitative real-time PCR showed that GhGSTF1 and GhGSTF2were highly expressed in red leaves and stems of Gossypium hirsutum L. T586.

    Article Title: Nano chitosan–zinc complex improves the growth performance and antioxidant capacity of the small intestine in weaned piglets
    Article Snippet: The present study was conducted to test the hypothesis that dietary supplementation with a nano chitosan-zinc complex (CP-Zn, 100 mg/kg zinc) could alleviate weaning stress in piglets challenged with ETEC K88 by improving growth performance and intestinal antioxidant capacity.. The in vivo effects of CP-Zn on growth performance variables (including gastrointestinal digestion and absorption functions and the levels of key proteins related to muscle growth) and the antioxidant capacity of the small intestine were evaluated in 72 weaned piglets.. The porcine jejunal epithelial cell line IPEC-J2 was used to further investigate the antioxidant mechanism of CP-Zn in vitro.

    Article Title: Effects of maternal T-2 toxin exposure on microorganisms and intestinal barrier function in young mice.
    Article Snippet: Real-time PCR was conducted on the QuantStudio® 5 Real-Time PCR Instrument (Applied Biosystems, Thermo Fisher Scientific, Wilmington, DE, USA) with the SYBR Green I (TSINGKE BioTech Co., Ltd., Beijing, China).

    Article Title: BST2 regulated by the transcription factor STAT1 can promote metastasis, invasion and proliferation of oral squamous cell carcinoma via the AKT/ERK1/2 signaling pathway.
    Article Snippet: Quantitative PCR assays were performed using a A28134 QuantStudio® 5 Real‐Time PCR Instrument (Thermo Fisher Scientific, Inc.) and iTaq Universal SYBR Green Supermixi reagent (Bio‐Rad Laboratories, Inc.) in 8 Strip Real‐time PCR Tubes (Sangon Biotech Co., Ltd.).

    Article Title: Fine particulate matter from pig house induced immune response by activating TLR4/MAPK/NF-κB pathway and NLRP3 inflammasome in alveolar macrophages.
    Article Snippet: The concentration, morphology and chemical components of PM2.5 were investigated.. PM2.5 can activate TLR4 and NLRP3 inflammasome in 3D4/21 cells.. MAPK and NF-kB pathways participated in NLRP3 inflammasome

    Article Title: The effects of indole-2,3-dione on radiosensitivity of U251 cells and zebrafish embryos.
    Article Snippet: Radiotherapy is an effective treatment for gliomas, which are among the most malignant tumors in the central nervous system (CNS).. A significant challenge in glioma radiotherapy is improving tumor control while minimizing damage to normal nervous tissue.. In this study, we investigated the dual role of indole-2,3-dione (Isatin, ISA), a compound with diverse biological and pharmacological activities, including anticancer, antioxidant, and anti-inflammatory properties, in modulating radiosensitivity.

    Article Title: Combined transcriptome and metabolome analysis provides insight into the ERF073 - malic acid network in pakchoi under submergence stress
    Article Snippet: The PCR reaction with TransStart ® Top Green qPCR SuperMix (+ Dye I) (Transgene, China) was carried out using a QuantStudio® 5 Real-time PCR Instrument (Thermo Fisher Scientific).



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